Journal: International Journal of Nanomedicine

Article Title: In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes

doi: 10.2147/IJN.S53432

Figure Lengend Snippet: Fluorescence microscopy of bone-marrow-derived DC 48 hours post-transfection with pEGFP-N1 using plain-L or TATp-L as carrier. Notes: Bright field ( A ), Hoechst ( B ), EGFP ( C ), ( B ) and ( C ) merged ( D ). Reference marker =10 μm. Abbreviations: DC, dendritic cells; plain-L, plain liposomes; pEGFP-N1, plasmid encoding for the enhanced green fluorescent protein; TATp-L, TAT peptide liposomes.

Article Snippet: A transfection grade purification Plasmid Mega Kit was purchased from Qiagen (Valencia, CA, USA).

Techniques: Fluorescence, Microscopy, Derivative Assay, Transfection, Marker, Plasmid Preparation

Journal: International Journal of Nanomedicine

Article Title: In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes

doi: 10.2147/IJN.S53432

Figure Lengend Snippet: EGFP transfection efficiency. Notes: The percentage of transfected cells was counted 48 hours post-transfection. EGFP was expressed at a higher value in DC treated with TATp-L (67%±5.0%) in comparison with plain-L (31%±6.9%). *** P <0.0001, Student’s t -test, n=6 ( A ). Double analysis of EGFP expression and CD11c specific marker resulted in 47% for TATp-L and 22% for plain-L ( B ). Abbreviations: DC, dendritic cells; EGFP, enhanced green fluorescent protein; PerCP-Cy5.5, peridinin chlorophyll cyanine 5.5; plain-L, plain liposomes; TATp-L, TAT peptide liposomes.

Article Snippet: A transfection grade purification Plasmid Mega Kit was purchased from Qiagen (Valencia, CA, USA).

Techniques: Transfection, Expressing, Marker

Journal: International Journal of Nanomedicine

Article Title: In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes

doi: 10.2147/IJN.S53432

Figure Lengend Snippet: IL-6 secretion by dendritic cells 48 hours post-transfection. Notes: Cytokines were assayed in DC culture supernatants 48 hours post-transfection by ELISA. IL-6 was expressed at a high value only from TATp-L-treated cells. pCIneo: pCIneo plasmid (empty, as control). pCIgD A : pCIgD A plasmid (encoding for BoHV-1 gD anchored version). *** P <0.001 for multiple comparisons in a Bonferroni post hoc test (against all), n=6. Abbreviations: BoHV-1, bovine herpes virus type 1; DC, dendritic cells; ELISA, enzyme-linked immunosorbent assay; gD, glycoprotein D; IL-6, interleukin 6; pCIneo, pCI-neo plasmid not encoding for an antigen; pCIgD A , pCI-neo plasmid encoding for BoHV-1 anchored gD; TATp-L, TAT peptide liposomes.

Article Snippet: A transfection grade purification Plasmid Mega Kit was purchased from Qiagen (Valencia, CA, USA).

Techniques: Transfection, Enzyme-linked Immunosorbent Assay, Plasmid Preparation